Chromatin |
MNase Digestion for Native ChIP
ChIP Using Chelex
Histone Purification on FPLC
Isolation of Oligonucleosomes by MNase Digestion
X-ChIP Protocol with LM-PCR Amplification (for microarray analysis)
HMT Assay
Chromatin Fibers From Mammalian Tissue Culture Cells
ChIP Using Chelex
Histone Purification on FPLC
Isolation of Oligonucleosomes by MNase Digestion
X-ChIP Protocol with LM-PCR Amplification (for microarray analysis)
HMT Assay
Chromatin Fibers From Mammalian Tissue Culture Cells
Immunoprecipitation
Ammonium Sulfate IgG Precipitation
Nucleosome Immunoprecipitation
Batch Crosslinking of Antibody to Protein G Sepharose
Nucleosome Immunoprecipitation
Batch Crosslinking of Antibody to Protein G Sepharose
Immunofluorescence
In vivo
Freezing Cells for Long Term Storage
Cell Cycle Synchronization
Transient Transfection by Calcium Phosphate
Luciferase Assay
Lentivirus Production and Infection
Propidium Iodide Staining for FACS
Protein Lysate
Cell Cycle Synchronization
Transient Transfection by Calcium Phosphate
Luciferase Assay
Lentivirus Production and Infection
Propidium Iodide Staining for FACS
Protein Lysate
Molecular Techniques
SDS-PAGE—Laemmli
Western Blot
Real-Time PCR with the iCycler5
Annealing Two Single-Stranded Oligos
cDNA from Muscle RNA
Western Blot
Real-Time PCR with the iCycler5
Annealing Two Single-Stranded Oligos
cDNA from Muscle RNA