Judd Rice Laboratory
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Chromatin

MNase Digestion for Native ChIP
ChIP Using Chelex
Histone Purification on FPLC
Isolation of Oligonucleosomes by MNase Digestion
X-ChIP Protocol with LM-PCR Amplification (for microarray analysis)
HMT Assay
Chromatin Fibers From Mammalian Tissue Culture Cells
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Immunoprecipitation

Ammonium Sulfate IgG Precipitation
Nucleosome Immunoprecipitation
Batch Crosslinking of Antibody to Protein G Sepharose

Immunofluorescence

Immunofluorescence

In vivo

Freezing Cells for Long Term Storage
Cell Cycle Synchronization
Transient Transfection by Calcium Phosphate
Luciferase Assay
Lentivirus Production and Infection
Propidium Iodide Staining for FACS
Protein Lysate

Molecular Techniques

SDS-PAGE—Laemmli
Western Blot
Real-Time PCR with the iCycler5
Annealing Two Single-Stranded Oligos
cDNA from Muscle RNA

Stock Solutions

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